Chilled and post-thawed semen characteristics of buffalo semen diluted in tris extender enriched with date palm pollen grains (TPG)

P-137: Effect of Different Equilibration, Freezing and Thawing Rate on Cryopreserved Buffalo Spermatozoa Diluted inTris- Egg Yolk Extender

Background: Artificial insemination (AI) has been used for increasing genetic potential of animals in production while application of AI has been restricted in buffalo due to low freezing ability and fertility of frozen-thawed buffalo spermatozoa. So improvement in buffalo sperm cryopreservation may enhance the breeding programs of this valuable farm animal. Semen processing is one of the major...

Association of oxidative status and semen characteristics with seminal plasma proteins of buffalo semen

To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO),...

Quality of Bovine Chilled or Frozen-Thawed Semen after Addition of Omega-3 Fatty Acids Supplementation to Extender

Effects of Various Cryoprotective Agents and Extender Osmolality on Post-thawed Ram Semen

The influence of different extender osmolality levels and the presence of different cryoprotectants on the postthawed semen’s characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the cryoprotectants: 6% glycerol, 6% 1,2propanediol, 6...

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...